Contact an Account ManagerSimultaneous determination of atropine and its acidic and basic degradation product by mixed-column HPLC
Summary
Type: HPLC
Application #: 101280
Catalog#: 720095.46 - MN HPLC Column, Nucleosil NH2, 100A, 5um, 4.6x250mm
Detection: UV 254 nm
Conditions: Used coluns:
a) 5cm NUCLEOSIL® 100-5 CN and 5cm4.65mm NUCLEOSIL® 100-5 NH2 in series,
(b) 4.4cm containing a mixture of NUCLEOSIL® 100-5 CN and NUCLEOSIL® 100-5NH2 (2:1, w/w),
(c) 15cm NUCLEOSIL® 100-5 CN and 5cm NUCLEOSIL® 100-5 NH2 in series, and
(d) 5cm NUCLEOSIL® 100-5 NH2 and 10cm NUCLEOSIL® 100-5 CN with column switching.
Eluent: 0.05M sodium acetate buffer pH 5 - methanol (3:1, v/v)
Authors: Lund U
Source: J Chromatogr 161 (1978) 371 - 378
Details
Legend: Separation of atropine and degradation products: (sample volume 2µl)
AP = atropine sulphate, 36.8mg/ml,
AAP = apoatropine hydrochloride, 0.12 mg/ml,
TA = tropic acid, 1.96mg/ml,
ATA = atropic ac